Anti-wrinkle cosmetic composition, composition system and method for cosmetic skin treatment

ABSTRACT

The present invention relates to gel cream anti-aging cosmetic compositions in the form of an oil-in-water emulsion comprising sensory ingredients that are suitable for the complete treatment of the skin and also provide specific benefits for day and night care, which complement each other.

FIELD OF THE INVENTION

The present invention relates to gel cream anti-aging cosmeticcompositions in the form of an oil-in-water emulsion comprising sensoryingredients that are suitable for the complete treatment of the skin andalso provide specific benefits for day and night care, which complementeach other.

PRIOR ART

Skin aging is the result of several factors. In addition to theindividual genetic predisposition, there are external factors and style-and quality of life-related factors that cause our organism, includingthe skin, to undergo changes and decelerations of various physiologicalprocesses.

It is known that after the age of thirty (30) six major physiologicalmechanisms of the skin are impaired, it being possible to notice somesigns of skin aging caused by the deceleration of some physiologicalprocesses.

The affected mechanisms are:

-   1. cell renewal;-   2. skin defense systems against free radicals, which can cause loss    of cellular energy;-   3. repeated facial movements cause the formation of creases from    micro tensions on the skin;-   4. loss of the natural moisturizing barrier of the skin;-   5. reduction in the elastic system precursors of the skin; and-   6. reduction in skin collagen precursors.

After the age of forty-five (45), seven (7) physiological mechanisms ofthe skin are impaired:

-   1. increased reduction of collagen production and density;-   2. reduction in precursors and disarrangement of collagen fibers;-   3. glycation (stiffening) of collagen fibers;-   4. increase of micro-lesions that cause wrinkles;-   5. increased elastin breakdown;-   6. loss of the natural moisturizing barrier of the skin; and-   7. reduction in the elastic system precursors of the skin; and

After the age of sixty (60), there are also seven (7) physiologicalmechanisms of the skin that are impaired:

-   1. Increased reduction in hyaluronic acid production, in addition to    loss of density and disarrangement of the extracellular matrix    fibers;-   2. Increased reduction and disarrangement of the major elastic    structures;-   3. loss of functionality of the deeper layers of the skin;-   4. increased breakdown of the extracellular matrix fibers;-   5. loss of the natural moisturizing barrier of the skin;-   6. reduction in the elastic system precursors of the skin; and-   7. reduction in skin collagen precursors.

Ultimately, at the age of seventy (70), the seven (7) impairedphysiological mechanisms of the skin are:

-   1. Increased reduction of the thickness of the skin causing it to be    more fragile;-   2. loss of skin barrier functionality;-   3. reduction in the skin nutrients;-   4. reduction in the skin's defense mechanisms.-   5. reduction in the elastic system precursors of the skin;-   6. reduction in skin collagen precursors; and-   7. loss of the natural moisturizing barrier of the skin.

From among these external factors, environmental conditions considerablyinfluence the body's functioning in general, since the human body has arhythmic behavior, running cycles associated with the time (e.g., dayand night).

In chronobiology (a science that studies biological phenomena from thechronological point of view) there are several rhythms associated withtheir length of time, including the circadian rhythm, which cycles arecompleted every 24 hours.

The skin does not “perceive” variations in light, however, it is subjectto environmental agents such as temperature, moisture, mechanical andbiological agents.

Some skin mechanisms present more robust scientific information on dailyvariations, for example:

Transepidermal water loss, subcutaneous blood flow intensity and aminoacid content in the skin: these phenomena are much more marked at night;

Sebaceous glands secretion: the production of tallow and fat is greaterduring the day with its maximum peak at noon;

Skin temperature: the skin temperature varies according to the measuredsite; the skin temperature on the forearm achieves its maximum in thelate afternoon, while on the face it reaches its maximum in the morning;at night, the skin temperature is lower.

Cell proliferation capacity that is higher around 11 PM and minimumaround 12 PM.

Cellular healing mechanisms: most of the mapped genes related to suchmechanism reach their peak activity during the day;

Leukocyte differentiation and cytokine production for immune response:most of the mapped genes related to such mechanism reach their peakactivity during the day.

Therefore, the effect of a treatment may vary and may be dependent onthe time of administration. Factors such as absorption, metabolizationcapacity, storage and affinity of a compound to cellular receptors mayvary according to circadian rhythms.

To renew and recover the skin energy, it is not sufficient to treat justa single cause of the problem, but to act on all of them. To that end,an anti-aging treatment must act on the maintenance, stimulation andmore effective regulation of the production of substances thatconstitute the skin, more intense cell renewal, recovery of naturalmoisturization combined with a stronger prevention against the mainaggression mechanisms.

Existing products take care of only a few specific points, but not allthe above mechanisms at the same time.

There is therefore a need for a composition acting on all thesemechanisms simultaneously, providing an effective skin care treatment.

SUMMARY OF THE INVENTION

The present invention relates to anti-aging cosmetic compositions in theform of an oil-in-water emulsion, comprising:

a) at least one emollient;

b) at least one antioxidant;

c) at least one humectant;

-   d) at least one active ingredient;-   e) at least one emulsifier;

f) at least one sensory modifier; and

g) cosmetically acceptable carriers.

Another object of the present invention relates to a composition systemcomprising a day care composition for and a night care composition.

A further object relates to a cosmetic treatment method of the skinwhich comprises applying the composition of the present invention.

BRIEF DESCRIPTION OF THE FIGURES

FIG. 1 shows a graph of the average variation in transepidermal waterloss from the skin obtained in the beginning of the study and after 7,14 and 28 days of use of (30+) compositions according to example 1 (daycare) over the control.

FIG. 2 shows a graph of the average variation in transepidermal waterloss from skin obtained in the beginning of the study and after 7, 14and 28 days of use of the (30+) compositions according to example 2(night care) over the control.

FIGS. 3 and 4 show the average improvement in tests carried outaccording to example 8 of the present specification for (45+) night carecompositions.

FIG. 5 shows the mean values of the Ur/Ue parameter obtained in thebeginning of the study and after 28 days of home use (mean±SD, n=25) for(45+) day care compositions.

FIG. 6 depicts the mean values of the Ur/Uf parameter obtained in theface and forearm regions in the beginning of the study and after 28 daysof home use for (45+) day care compositions.

FIG. 7 depicts the mean values of Ur/Ue parameter obtained in thebeginning of the study and after 28 days of home use (mean±SD, n=23) for(45+) night care compositions.

FIG. 8 shows the mean values of the Ur/Uf parameter obtained in thebeginning of the study and after 28 days of home use (mean±SD, n=23) for(45+) night care compositions.

FIG. 9 shows the average variation in transepidermal water loss from theskin obtained in the beginning of the study and after 7, 14 and 28 daysof use of the (45+) composition over control (Mean±SD, n=26).

FIG. 10 shows the hydration kinetics of product (45+) according to thepresent invention as compared to the control.

FIG. 11 shows the kinetics of the percentage of hydration given bycomposition (45+) relative to control.

FIGS. 12 and 13 show the percentage of improvement in the anti-agingefficacy of anti-aging (60+) cosmetic day care compositions according tothe present invention by means of instrumental measurements under normaluse conditions.

FIGS. 14 and 15 show the percentage of improvement in the anti-agingefficacy of anti-aging (60+) cosmetic night care compositions accordingto the present invention by means of instrumental measurements undernormal use conditions.

FIG. 16 shows the average variation in transepidermal water loss fromskin obtained in the beginning of the study and after 7, 14 and 28 daysof use of the (60+) compositions according to the present invention(lighter bars) over the control (darker bars), mean±SD, n=26.

FIGS. 17 and 18 show the percentage of improvement in the anti-agingefficacy of anti-aging (70+) cosmetic day care compositions according tothe present invention by means of instrumental measurements under normaluse conditions.

FIGS. 19 and 20 show the percentage of improvement in the anti-agingefficacy of anti-aging (70+) cosmetic night care compositions accordingto the present invention by means of instrumental measurements undernormal use conditions.

FIGS. 21 and 22 show the average variation in transepidermal water lossfrom the skin obtained in the beginning of the study and after 7, 14 and28 days of use of the composition (lighter bars) relative to the control(darker bars), Mean±SD, n=25, for day and night care (70+) compositions,respectively.

DESCRIPTION OF THE INVENTION

The present invention relates to gel cream anti-aging cosmeticcompositions in the form of an oil-in-water emulsion capable of treatingsimultaneously the six major physiological mechanisms of the skin,providing long-term benefits with progressive results over the period oftreatment.

The six major physiological mechanisms according to the presentinvention include:

-   1. cell renewal;-   2. protection of cellular energy;-   3. relaxing of skin micro tensions;-   4. recovery of the natural moisturizing barrier;-   5. elastin stimulation; and-   6. collagen stimulation.

Hereinafter, such mechanisms will be referred to as “the six majorphysiological mechanisms of the skin”.

By “skin” is meant skin from the neck, face, arm, forearm, chest andhand.

By “anti-aging cosmetic compositions” is meant cosmetic compositionssuitable for use in various age groups. Preferably, the age groupscomprise ages over 30 years, particularly over 45 years, over 60 yearsand over 70 years.

For ease of reference, specific compositions for distinct age groupswill be cited as: (30+) compositions referring to ages over 30 years;(45+) compositions referring to ages over 45 years; (60+) compositionsreferring to ages over 60 years; and (75+) compositions referring toages over 60 years.

Another object of the present invention consists of a system ofanti-aging, day care and night care cosmetic compositions, whichcomplement each other, having suitable active ingredients for eachperiod of the day.

Suitable active ingredients for each period in the present inventionrefer to are sunscreens, active ingredients having antioxidant activityand prolonged day-time hydration, and active ingredients for stimulatingskin regeneration by eliminating toxins and deep night-time nutrition.

Yet another object of the invention is to provide cosmetic anti-agingcompositions having a cream gel texture, which is easy to be applied onthe skin, forming an emollient and wetting film without leaving a shinyand oily appearance.

The cream gel texture and its qualities according to the presentinvention are obtained by using low and medium molecular weight estershaving high levels of volatile emollients that evaporate rapidly duringapplication providing greater spreadability and fast absorption.

Therefore, the present invention relates to cosmetic cream gelanti-aging compositions in the form of an oil-in-water emulsioncomprising at least one emollient, at least one antioxidant, at leastone humectant, at least one active ingredient, at least one emulsifier,at least one sensory modifier and cosmetically acceptable carriers.

Another object of the present invention is a method for the cosmetictreatment of the skin comprising the topical application of acomposition of the present invention on the skin to be treated, whereinthe application takes place during the day and/or at night.

The anti-aging cosmetic compositions of the present invention comprise,in particular:

-   a) at least one emollient;-   b) at least one antioxidant;-   c) at least one humectant;-   d) at least one active ingredient;-   e) at least one emulsifier;-   f) at least one sensory modifier; and-   g) cosmetically acceptable carriers.

Emollients, without limiting the scope of the present invention, may beselected from caprylyl methicone, C₁₂₋₁₅ alkyl benzoate, dibutyladipate, dicaprylyl carbonate, isononyl isononanoate, dicapryl ether,dodecane, ethyl hexyl palm itate, ethyl macadam ate, isohexadecane,capricicaprylic triglyceride, butters from the Brazilian biodiversity,isoamyl cocoate or mixtures thereof.

Without being intended to limit the scope of the present invention, thefollowing butters from the Brazilian bioversity may be used: murumurubutter which aids to replace skin barrier lipids providing repair andprotection and resulting in decreased loss of water and maintenance ofthe natural skin hydration; Further butters such as those obtained fromcocoa beans (Theobroma cacao), Cupuaçu (Theobroma grandiflorum), Ucuúbaand

Sapucainha, or mixtures thereof.

Butters aid in the lipid replacement of the skin barrier, providingrepair and protection, which helps to reduce the loss of water and tomaintain the natural skin hydration for longer periods of time.

Antioxidants, without being intended to limit the scope of the presentinvention, may be selected from butylated hydroxytoluene (BHT),tocopherol acetate or natural plant extracts, for example Camelliasinensis (green tea), Theobroma cacao (cocoa), Spilanthes acmella(Jambu) or mixtures thereof.

The polyphenols present in the green tea extract, mainly have theability to interact with cell membranes and protect cells from oxidativeprocesses, thus ensuring the proper functioning and protection of cellenergy, also promoting cell renewal.

Humectants, without limiting the scope of the present invention, may beselected from sugar alcohols, such as glycols, glycerol, sorbitol,mannitol or mixtures thereof.

Preferably, the humectant is vegetable glycerin extracted from palm oil,which aids in surface hydration. Such effect coupled with the protectionprovided by butters promotes recovery of the natural moisturizingbarrier of the skin.

The active ingredient of the present invention may be selected frompeptides which are in general synthetic molecules, particularlyacetyl-tetrapeptide-2, which acts on the stimulation of collagen andelastin production, thus promoting improved skin tone and elasticity.

The active ingredient may also be selected from Hymenaea courbarilextract; a mixture of sodium cocoyl amino acids/sarcosine/potassiumaspartate/magnesium aspartate/propylene glycol; a mixture of Paeoniaalbiflora extract/phenoxyethanolfethylhexylglycerin; Cichorium intybusextract; Schinus Terebinthifollus leaf extract; omega 6 passion fruitceramide; or mixtures thereof.

When Paeonia albiflora and Cichorium intybus extracts are used, rootextracts from these plants are particularly used.

Emulsifiers, without limiting the scope of the present invention, may beselected from at least one of glyceryl stearate citrate, potassiumcetylphosphate, PEG-100, acrylates, xanthan gum, cetearyl alcohol, amixture of glyceryl stearate/PEG-100, among others known in the art.state of the art, or mixtures thereof.

Sensory modifiers, without limiting the scope of the present invention,may be selected from silicones, such as cyclopentasiloxane, dimethiconeor cyclopentasiloxane/dimethicone crospolymers, or other compounds suchas titanium isopropyl triisostearate, nylon-12,polymethylsilsesquioxane, aluminum starch octenylsuccinate or mixturesthereof.

The compositions according to the present invention may further comprisea viscosity donor selected from acrylate/C₁₀₋₃₀ alkyl acrylate,carbopol, such as Carbopol ETD 2020, a mixture ofhydroxuethylacrilate/sodium acryloyldimethyltaurate copolymer, squaleneand polysorbate 60 (Simulgel NS), or mixtures thereof.

Cosmetically acceptable carriers may be selected from compounds known inthe art. Examples of carriers are: preservatives, perfumes/fragrances,polymer neutralizers, chelating agents, pH adjusters, among others.Disodium EDTA (chelating agent), iodopropynyl butylcarbamate(preservative), phenoxyethanol (preservative), pataqueira essential oil(perfume) and triethanolamine (pH adjusting agent) are particularlyused.

Sunscreens, without limiting the scope of the present invention, may beselected from bemotrizinol (bis-ethylhexyloxyphenol methoxyphenyltriazine), diethylaminohydroxybenzoylhexyl benzoate, ethylhexylmethoxycinnamate, homosalate, bisoctrizole (TINOSORB M), ethylhexyl triazone ormixtures thereof.

Actives for stimulating skin regeneration by eliminating toxins in thepresent invention relate to Candida saitoana extract; bisabolol;Casearia sylvestris leaf extract, and a mixture of Caseariasylvestris/silica or mixtures thereof.

Candida saitoana extract stimulates cellular detoxification mechanismsthrough cellular autophagy, thereby reducing the build-up of cellulartoxins.

Bisabolol, which may also be included in the composition, acts on thedermis and epidermis damage mechanisms by regulating the production ofmicrodamage causative agents IL-6 and IL-8.

The following examples, without limitation, illustrate the anti-agingcosmetic compositions according to the present invention, whichsurprisingly act simultaneously on the six major physiologicalmechanisms of the skin, promoting deep nutrition combined with theantioxidative effect and the effect of elimination of toxins,particularly when they are used in the form of a system day care andnight care compositions that complement each other.

EXAMPLES Example 1 Preparation of (30+) (45+) (60+) (70+) Day CareCosmetic Compositions

Day care cosmetic compositions were produced as oil-in-water cream gelemulsions where oil is the dispersed phase and water is the continuousphase. Both phases were heated at a temperature of 75-80° C., the oilyphase, where the sunscreens are, was subsequently poured onto theaqueous phase under stirring for about 10 minutes. Thereafter thecooling phase was started by adding an aqueous phase with a polymerneutralizing agent. When the temperature reached about 60° C., thesensory modifier phase (silicones) was added and when the temperaturereached 40° C., preservatives, fragrance, sensory modifiers (particles)and the high temperature-sensitive agents were added.

The following table illustrates the (30+) cosmetic compositions,according to the present invention, thus produced:

TABLE 1 (30+) anti-aging, day care cosmetic compositions IngredientExample A Example B Acetyl tetrapeptide-2 and caprylyl glycol 1.5 1 andwater complex Acrylates/C₁₀₋₃₀ Alkyl Acrylate 0.25 0.15 CrosspolymerAluminum Starch Octenylsuccinate 2 1.5 Butylated Hydroxytoluene (BHT)0.1 0.1 Bemotrizinol 2.95 3.2 Camellia sinensis 0.025 0.025 (leafextract) Caprylyl Methicone 1.5 1 Cyclopentasiloxane 9 9Cyclopentasiloxane (and) Dimethicone 3 2 Crosspolymer C₁₂₋₁₅ AlkylBenzoate 2 1 Dibutyl Adipate 0.5 0.6 Dicaprylyl Carbonate 2.5 2.4Diethylamino Hydroxybenzoyl 3 4.8 Hexylbenzoate Disodium EDTA 0.1 0.1Ethylhexyl Methoxycinnamate 9 13 fragrance 0.22 0.22 Glycerin 5 7.935Glyceryl Stearate Citrate 1.5 0.5 Homosalate 3 4 IodopropynylButylcarbamate 0.098 0.098 Murumuru butter 0.5 0.5 (crude/filtered)Pataqueira essential oil 0.001 0.001 Phenoxyethanol 0.8 0.8 PotassiumCetyl Phosphate 0.6 0.6 Simulgel NS 2 1 Spilanthes acmella 0.125 0.125Theobroma cacao (seed extract) 0.01 0.01 Tinosorb M 2.5 3 TocopherolAcetate 0.2 0.2 Triethanolamine 99W 0.35 0.5 xanthan gum 0.25 0.415 aqua45.421 40.221

The following table illustrates the (45+) cosmetic compositions,according to the present invention, thus produced:

TABLE 2 (45+) anti-aging, day care cosmetic compositions IngredientExample A Example B Acetyl tetrapeptide-2 and caprylyl glycol 1.50 1.50and water complex Acrylates/C₁₀₋₃₀ Alkyl Acrylate 0.28 0.20 Crosspolymeraqua 41.90 35.48 Butylated Hydroxytoluene (BHT) 0.10 0.10 Bemotrizinol2.95 2.74 Camellia sinensis leaf extract 0.01 0.01 caprylyl methicone1.50 1.20 Caprylic/Capric Triglyceride 3.00 2.40 Cyclopentasiloxane 9.0012.50 C₁₂₋₁₅ Alkyl Benzoate 2.00 2.80 Diethylamino hydroxybenzoyl hexyl2.50 1.50 benzoate Disodium EDTA 0.10 0.12 Ethylhexyl Methoxycinnamate9.00 7.80 Ethylhexyl Triazone 1.00 1.20 Fragrance 0.18 0.16 Glycerin5.00 4.00 Glyceryl Stearate Citrate (plant origin) 1.50 2.15 Homosalate3.00 4.70 Hymenaea courbaril (leaf extract) 0.25 0.41 IodopropynylButylcarbamate 0.10 0.15 Isoamyl Cocoate 2.00 3.20 Crude/FilteredMurumuru Butter 0.50 0.70 Nylon-12 1.00 1.50 Pataqueira essential oil0.001 0.001 Phenoxyethanol 0.80 0.80 Polymethylsilsesquioxane 4.50 3.70Potassium Cetyl Phosphate 0.60 0.50 Simulael NS 2.00 3.20 Sodium cocoylamino 1.50 2.20 acids/sarcosine/potassium aspartate/magnesiumaspartate/propylene glycol and water complex Theobroma cacao (leafextract) 0.01 0.01 Tinosorb M 1.50 2.00 Tocopherol Acetate 0.20 0.32Triethanolamine 0.38 0.55 Xanthan gum 0.15 0.20

The following table illustrates the (60+) cosmetic compositions,according to the present invention, thus produced:

TABLE 3 (60+) anti-aging, day care cosmetic compositions Example ExampleIngredient A B Acetyl tetrapeptide-2 and caprylyl glycol 1.50 2.00 andwater complex Acrylates/C₁₀₋₃₀ Alkyl Acrylate 0.30 0.40 CrosspolymerWater and Paeonia albiflora root 2.00 4.00extract/phenoxyethanol/ethylhexyl glycerin complex aqua 44.91 44.68Butylated Hydroxytoluene (BHT) 0.10 0.12 Bemotrizinol 2.95 4.00 Camelliasinensis leaf extract 0.01 0.01 caprylyl methicone 1.50 1.20Caprylic/Capric Triglyceride 3.00 2.00 Cyclopentasiloxane 8.00 5.00C₁₂₋₁₅ Alkyl Benzoate 2.00 1.00 Diethylamino Hydroxybenzoyl Hexyl 2.503.00 Benzoate Disodium EDTA 0.10 0.15 Ethylhexyl Methoxycinnamate 9.005.00 Ethylhexyl Triazone 1.00 1.50 Fragrance 1 0.20 0.21 Fragrance 20.0010 0.0015 Glycerin 5.00 7.00 Glyceryl Stearate Citrate 1.50 1.20Homosalate 3.00 2.00 Iodopropynyl Butylcarbarnate 0.10 0.08 IsoamylCocoate-TEGOSOFT AC 2.00 3.20 Crude/Filtered Murumuru Butter 0.50 0.75Phenoxyethanol 0.80 1.36 Polymethylsilsesquioxane 3.00 4.50 PotassiumCetyl Phosphate 0.60 0.50 Schinus terebinthifolius (leaf extract) 0.010.01 SIMULGEL NS 2.00 1.50 Theobroma cacao (cocoa) seed extract 0.010.01 Tinosorb M 1.50 2.55 Tocopherol Acetate 0.20 0.15 Triethanolamine0.38 0.40 Casearia sylvestris leaf extract 0.05 0.07 Xanthan gum 0.280.45

The following table illustrates the (70+) cosmetic compositions,according to the present invention, thus produced:

TABLE 4 (70+) anti-aging, day care cosmetic compostions IngredientsExample A Example B Acetyl tetrapeptide-2 and caprylyl 0.45 2.55 glycoland water complex Acrylates/C₁₀₋₃₀ Alkyl Acrylate 0.09 0.51 CrosspolymerWater and Cichorium intybus root 0.9 5.1 extract complex aqua 13.057273.9908 Butylated Hydroxytoluene (BHT) 0.03 0.17 Bemotrizinol 0.8855.015 Camellia sinensis leaf extract 0.003 0.017 caprylyl methicone 0.452.55 Caprylic/Capric Triglyceride 0.9 5.1 Cvclopentasiloxane 2.4 13.6C₁₂₋₁₅ Alkyl Benzoate 0.6 3.4 Diethylamino Hydroxybenzoyl Hexyl 0.754.25 Benzoate Disodium EDTA 0.03 0.17 Ethylhexyl Methoxycinnamate 2.715.3 Ethylhexyl Triazone 0.3 1.7 Fragrance 0.105 0.595 Glycerin 1.5 8.5Glyceryl Stearate Citrate 0.45 2.55 Homosalate 0.9 5.1 IodopropynylButylcarbamate 0.0294 0.1666 Isoamyl Cocoate-TEGOSOFT AC 0.6 3.4Murumuru butter 0.15 0.85 Pataqueira essential oil 0.0009 0.0051 Omega 6passion fruit ceramide 0.09 0.51 Phenoxyethanol 0.24 1.36Polymethylsilsesquioxane 0.9 5.1 Potassium Cetyl Phosphate 0.18 1.02SIMULGEL NS 0.6 3.4 Theobroma cacao seed extract 0.003 0.017 TINOSORB M0.45 2.55 Tocopherol Acetate 0.06 0.34 Triethanolamine 0.114 0.646Xanthan gum 0.0825 0.4675

Example 2 Preparation of (30+) (45+) (60+) (70+) Night Care Compositions

Night care cosmetic compositions were produced as oil-in-water cream gelemulsions where oil is the dispersed phase and water is the continuousphase. In this case, heating of the aqueous phase was started in themain container to the temperature of about 75 and about 80 ° C. and,with stirring, the oily phase was added under stirring for about 10minutes. Thereafter the cooling phase was started by adding an aqueousphase with a polymer neutralizing agent. When the temperature reachedabout 60° C., the sensory modifier phase (silicones) was added and whenthe temperature reached about 40° C., preservatives, fragrance, sensorymodifiers (particles) and high temperature-sensitive agents were added.

The following table illustrates the (30+) cosmetic compositionsaccording to the present invention thus produced:

TABLE 5 (30+) anti-aging, night care cosmetic compositions IngredientExample C Example D Acetyl tetrapeptide-2 and caprylyl 2 3 glycol andwater complex Butylated Hydroxytoluene (BHT) 0.1 0.15 Bisabolol 0.5 0.7Camellia sinensis (leaf extract) 0.025 0.0125 Carbopol ETD 2020 0.250.125 Cyclopentasiloxane 6 10 Cyclopentasiloxane (and) Dimethicone 4 2Crosspolymer C₁₂₋₁₅ Alkyl Benzoate 2 1 Dicaprylyl Carbonate 3.5 1.75Disodium EDTA 0.1 0.05 fragrance 0.22 0.11 Glycerin 5 6.142 Glycerylstearate and PEG-100 Stearate 1 0.5 Candida saitoana (hydrolyzedextract) 1.65 0.825 Iodopropynyl Butylcarbamate 0.098 0.049 IsononylIsononanoate 1 0.5 Nylon1212 (SP-10L) 3.5 5 Pataqueira essential oil0.001 0.0015 Phenoxyethanol 0.8 1.2 Simulgel NS 3 4.2 Spilanthes acmella0.125 0.13 Theobroma cacao (seed extract) 0.01 0.015 Theobroma cacao(seed butter) 0.5 0.7 Theobroma grandiflorum 0.5 0.45 (seed butter)Tocopherol Acetate 0.2 0.31 Triethanolamine 99W 0.35 0.582 Xanthan gum0.25 0.345 aqua 63.321 60.153

The following table illustrates the (45+) cosmetic compositionsaccording to the present invention thus produced:

TABLE 6 (45+) anti-aging, night care cosmetic compositions IngredientExample C Example D Acetyl tetrapeptide-2 and caprylyl 2.00 3.20 glycoland water complex aqua 64.291 58.991 Butylated Hydroxytoluene (BHT) 0.100.05 Bisabolol 0.50 0.43 Carbopol ETD 2020 0.25 0.37 Cetearyl Alcohol1.00 0.60 Cyclopentasiloxane 4.00 4.80 Cyclopentasiloxane (and)Dimethicone 4.00 5.70 Crosspolymer C₁₂₋₁₅ Alkyl Benzoate 2.00 3.00Dicaprylyl Carbonate 3.50 4.50 Disodium EDTA 0.10 0.10 Fragrance 0.200.20 Glycerin 5.00 5.00 Glyceryl stearate and PEG-100 Stearate- 1.201.20 plant origin Hydrolyzed Candida saitoana extract 1.65 1.65 Hymenaeacourbaril (leaf extract) 0.25 0.25 Iodopropynyl Butylcarbamate 0.0980.098 Isononyl Isononanoate 1.00 1.00 Nylon 12 3.00 3.00 Pataqueiraessential oil 0.001 0.001 Phenoxyethanol 0.80 0.80 Simulgel NS 2.00 2.00Sodium cocoyl amino 1.50 1.50 acids/sarcosine/potassiumaspartate/magnesium aspartate/propylene glycol and water complexTheobroma cacao seed extract 0.01 0.01 Theobroma cacao seed butter 0.500.50 Theobroma grandiflorum seed butter 0.50 0.50 Tocopherol Acetate0.20 0.20 Triethanolamine 0.35 0.35

The following table illustrates the (60+) cosmetic compositionsaccording to the present invention thus produced:

TABLE 7 (60+) anti-aging, night care cosmetic compositions IngredientExample C Example D Acetyl tetrapeptide-2 and caprylyl 2.00 1.50 glycoland water complex Water and Paeonia albiflora root 2.00 1.50extract/phenoxyethanol/ethylhexyl glycerin complex aqua 64.98 62.22Butylated Hydroxytoluene (BHT) 0.10 0.05 Bisabolol 0.50 0.40 CarbopolETD 2020 0.25 0.35 Cetearyl Alcohol 1.25 1.50 Cyclopentasiloxane 4.006.00 Cyclopentasiloxane (and) Dimethicone 4.00 6.50 CrosspolymerDicaprylyl Carbonate 1.00 1.50 Disodium EDTA 0.10 0.12 Ethyl Macadamate3.00 4.00 Fragrance 0.20 0.15 Glycerin 8.00 5.00 Glyceryl Stearate andPEG-100 1.20 1.00 Hydrolyzed Candida saitoana extract 1.65 1.20Iodopropynyl Butylcarbamate 0.10 0.05 Isononyl Isononanoate 1.00 1.50Pataqueira essential oil 0.0010 0.0015 Phenoxyethanol 0.80 1.20 Schinusterebinthifolius (leaf extract) 0.01 0.01 SIMULGEL NS 2.00 2.50Theobroma cacao (leaf extract) 0.01 0.01 Theobroma cacao seed butter0.50 0.30 Theobroma grandiflorum seed butter 0.50 0.40 TocopherolAcetate 0.20 0.15 Triethanolamine 0.35 0.50 Complex of water and amixture of 0.05 0.04 Casearia Sylvestris leaf extract and Silica Xanthangum 0.25 0.35

The following table illustrates the (70+) cosmetic compositionsaccording to the present invention thus produced:

TABLE 8 (70+) anti-aging, night care cosmetic compositions IngredientExample C Example D Acetyl tetrapeptide-2 and caprylyl 2.00 3.20 glycoland water complex Water and Cichorium intybus root 3.00 3.50 extractcomplex aqua 62.94 53.94 Butylated Hydroxytoluene (BHT) 0.10 0.12Bisabolol 0.50 0.75 Carbopol ETD 2020 0.25 0.30 Cetearyl Alcohol 2.002.80 Cyclopentasiloxane 4.00 5.80 Cyclopentasiloxane (and) Dimethicone4.00 3.00 Crosspolymer Dicaprylyl Carbonate 1.00 1.20 Disodium EDTA 0.100.15 Ethyl Macadamate 3.00 3.50 Fragrance 0.25 0.28 Glycerin 8.00 9.70Glyceryl Stearate and PEG-100 1.20 1.10 Hydrolyzed Candida saitoanaextract 1.65 1.7800 Iodopropynyl Butylcarbamate 0.10 0.13 IsononylIsononanoate 1.00 1.50 Pataqueira essential oil 0.00 0.0014 Omega 6passion fruit ceramide 0.30 0.42 Phenoxyethanol 0.80 1.24 SIMULGEL NS2.00 3.20 Theobroma cacao seed extract 0.01 0.0150 Theobroma cacao seedbutter 0.50 0.65 Theobroma grandiflorum seed butter 0.50 0.85 TocopherolAcetate 0.20 0.25 Triethanolamine 0.35 0.42 Xanthan gum 0.25 0.20

Example 3 Evaluation of Skin Hydration by Corneometry-Compositions (30+)

Volunteers have been recruited for an evaluation of the hydration effectof cosmetic compositions according to example 1 of the presentinvention. Twenty-one (21) survey participants have completed the studyand there were no reports or evidence of adverse reaction during thestudy.

The survey participants were asked to discontinue the use of anycosmetic product on their forearms up to 48 hours prior to the beginningof the study.

For the evaluation two 2.5×4.0 cm sites were marked on the volar forearmof the survey participant, wherein one site was used as control (withoutany products being applied). After the baseline corneometry measurements(performed on Corneometer® 825 and Multiprobe Adapter MPA-5,CKeletronics, Germany), the formulations according to example 1 wereapplied and the survey participants remained in the lab for additionalmeasurements to be taken after 15 minutes, 4.6, 8 and 12 hours.

After the 12-hour measurement the survey participants were sent home,being instructed not to wet or wash their arms. The next day theyreturned to the lab for another measurement to be taken 24 hours afterapplication.

Application of the compositions according to example 1 of the presentinvention was found to maintain the skin hydrated for up to 24 hours ascompared to control (skin without any products being applied).Application of the compositions according to example 1 of the presentinvention increased the skin hydration level by up to 62%. All theparticipants presented an improvement in skin hydration.

Example 4 Evaluation of the Anti-Aging Efficacy of (30+) Day CareCompositions through Instrumental Measurements Under Normal UseConditions

A study was carried out to ascertain the efficacy of the formulations ofExample 1 according to the present invention in reducing wrinkles andimproving skin texture.

The volunteers rested in a temperature- and moisture-controlled room for30 minutes before the baseline measurements and during the intervalbetween measurements. Application was made once a day on the face andneck evenly after cleansing the skin.

On the first day, after 14 days, and after 28 days, 7 consecutive imagesof the periorbital region were obtained using Optical 3D Skin MeasuringDevice PRI MOS Compact 5.075 for evaluation of wrinkles/texture on oneside of the face and 3 (front and side) images using Visia CR (CanfieldScientific, Inc.) that were used for registration purposes.

Analysis of the obtained images allowed us to conclude that thecompositions according to example 1 of the present invention caused areduction in the wrinkle volume, a reduction in the average depth of thewrinkles and a reduction in the maximum roughness of the wrinkles afterfourteen days of use.

Example 5 Evaluation of the Anti-Aging Efficacy of (30+) Night CareCosmetic Compositions through Instrumental Measurements Under Normal UseConditions

The cosmetic compositions evaluated herein are according to Example 2 ofthe present invention, the methodology used is the same as Example 4,except for the fact that measurements were taken on the first day, after14 days, after 28 days and after 56 days.

Analysis of the obtained images allowed us to conclude that the cosmeticcompositions according to example 2 of the present invention caused areduction in wrinkle volume, a reduction in the average depth of thewrinkles and an improvement in skin undulation after fifty-six days ofuse.

Example 6 Evaluation of the skin barrier fortifying effect provided bythe use of (30+) day care cosmetic compositions

A study was carried out to assess the effect of the compositionsaccording to example 1 of the present invention on the fortification ofthe skin barrier.

Volunteers were instructed to discontinue the use of any topicalproducts on their forearms for 48 hours prior to the beginning of thestudy.

The methodology consisted of assessing the transepidermal water lossfrom the skin after a process of partially removing the comeous extract.Measurements were collected in the beginning of the study and after 7,14 and 28 days of use of the compositions according to example 1 of thepresent invention.

A tape-stripping process was used to assess the effect of fortificationof the skin barrier, where a transparent medical adhesive tape wasapplied and removed 30 times repeatedly on sites marked on the volarforearm, followed by measuring the transepidermal water loss (Tewameter®300 and Multiprobe Adapter MPA-5, CKeletronics, Germany). Application ofthe compositions is made on only one forearm, causing the other to bethe control, that is, no topical products are applied thereto.

From the crude TEWL (trans epidermal water loss) values, designated E,the variation in transepidermal water loss was calculated as a functionof the removal of stratum corneum layers (ΔE).

ΔEi,X=ET30,X−E0,X

wherein: ΔE=Variation in transepidermal water loss from the skin; i=0,7, 14 or 28 days. ET30=TEWL value after 30 removals of stratum corneumlayers. ET0=TEWL value measured on whole skin; X=control or formulationused.

Analysis of the results is shown in the graph shown in FIG. 1 .

The obtained results allow us to conclude that the formulations had asignificant effect on skin barrier fortification as compared to controlafter 14 and 28 days of home use.

Example 7 Evaluation of the Skin Barrier Fortifying Effect Provided bythe Use of (30+) Night Care Compositions

The formulations to be evaluated are in accordance with Example 2 of thepresent invention, the methodology employed is the same as Example 6.Measurements and calculations were performed similarly to the previousexample.

Analysis of the results is shown in the graph shown in FIG. 2 .

The obtained results allow us to conclude that the formulations had asignificant effect on skin barrier fortification as compared to controlafter 14 and 28 days of home use.

Example 8 Evaluation of the Anti-Aging Efficacy of a (45+) Night CareCosmetic Product through Instrumental Measurements Under Normal UseConditions

The aim of the study was to ascertain the efficacy of compositionsaccording to the present invention in reducing wrinkles and improvingskin texture when applied once a day by instrumental evaluations after14±2 days, 28±2 days and 56±2 days of use.

35 female participants aged 46 to 59 years, average age of 53 years,phototypes Ito IV, having wrinkles or expression lines on theperiorbital region and of all skin types were evaluated by adermatologist in the beginning and in the end of the research. Thesurvey participants rested in a temperature- and moisture-controlledroom for 30 minutes before the baseline measurements and during theinterval between measurements. On D0, D14, D28 and D56, 7 consecutiveimages of the periorbital region were obtained using Optical 3D SkinMeasuring Device PRIMOS Compact 5.075 for evaluating wrinkles/texture onone side of the face and 3 (front and side) images using Visia CR(Canfield Scientific, Inc.) that were used for registration purposes.

According to the methodology used to assess efficacy, it was concludedthat relatively to the baseline (D0):

there was a reduction in wrinkle volume after twenty-eight days of use;

there was a reduction in the average roughness of the wrinkles afterfourteen, twenty-eight and fifty-six days of use;

there was a reduction in the average depth of the wrinkles afterfourteen and fifty-six days of use;

there was a reduction in wrinkle roughness after fourteen and fifty-sixdays of use;

there was an improvement in skin texture after fifty-six days of use.

FIGS. 3 and 4 show the above results.

Example 9 Efficacy Test—Clinical Trial Evaluating the Efficacy of (45+)Day Care Compositions in Reducing Signs of Facial Aging

Efficacy of the compositions according to the present invention inreducing signs of facial aging such as wrinkles and sagging wasevaluated by means of clinical and subjective evaluations performed on69 volunteers who applied the compositions once a day on the face andneck, evenly after cleansing the skin.

It was a single-center, non-comparative, non-randomized study intendedto assess the clinical efficacy in reducing the signs of facial aging ofa cosmetic product evaluated on the indicated site according to the modeof use for 28 days. Clinical assessments were performed on Visits: Visit01/D-7, Visit 02/D0, Visit 03/D07, Visit 04/D14, Visit 05/D28 and Visit061D56 after 56 days of use of the compositions.

Clinical evaluation times were: D-7 (start of wash out); D0 (prior tothe use of the product and after the wash-out period) D7 (after 7 daysof use of the product), D14 (fourteen days of use of the product), D28(twenty-eight days of use of the product) and D56 (fifty-six days of useof the product). Subjective evaluations were also performed on thevisits.

Clinical assessment has shown that:

The compositions according to the present invention provided animprovement in the facial contour at all evaluated times, beingstatistically significant (p≤0.05) at D56 as compared to the baselineD0;

The compositions according to the present invention provided animprovement in the healthy appearance of the skin of volunteers at allevaluated times, being statistically significant (p≤0.05) as compared tothe baseline D0;

The compositions according to the present invention provided animprovement in the general appearance of the skin of volunteers at allevaluated times, being statistically significant (p≤0.05) at D14 and D56as compared to the baseline D0;

In the subjective assessment:

There was an improvement in facial harmony (improvement in the facialcontour) of the volunteers' skin at all evaluated times, also beingstatistically significant (p≤0.05);

There was an improvement in the general appearance of the skin (bright,revitalized, rebalanced, healthy looking skin) of the volunteers at allevaluated times, also being statistically significant (p≤0.05);

It was observed that at least 82% of the volunteers reported that facialharmony and overall skin appearance were improved or were greatlyimproved after using the composition.

Example 10 Efficacy Test—Clinical Trial Evaluating the Efficacy of (45+)Night Care Compositions in Reducing Signs of Facial Aging

Efficacy of the compositions according to the present invention inreducing signs of facial aging such as wrinkles and sagging wasevaluated by means of clinical and subjective assessments performed on72 volunteers who used the compositions on a daily basis by evenlyapplying it on the face overnight after cleansing the skin.

It was a single-center, non-comparative, non-randomized study intendedto assess the clinical efficacy in reducing the signs of facial aging ofthe product evaluated on the indicated site according to the mode of usefor 28 days. Clinical assessments were performed on Visits: Visit01/D-7, Visit 02/D0, Visit 03/D07, Visit 04/D14, Visit 05/D28 and Visit06/D56 after 56 days of use of the compositions.

Clinical evaluation times were: D-7 (start of wash out); D0 (prior tothe use of the product and after the wash-out period) D7 (after 7 daysof use of the product), D14 (fourteen days of use of the product), D28(twenty-eight days of use of the product) and D56 (fifty-six days of useof the product). Subjective evaluations were also performed on thevisits.

Clinical assessment has shown that:

The compositions according to the present invention provided animprovement in the degree of periorbital wrinkles at D14, D28 and D56but they were not statistically significant (p>0.05);

The compositions according to the present invention provided animprovement in the facial contour at all evaluated times, beingstatistically significant (p≤0.05) at D56 as compared to the baselineD0;

The compositions according to the present invention provided animprovement in the healthy appearance of the skin of volunteers at allevaluated times, being statistically significant (p≤0.05) at D14, D28and D56 as compared to the baseline D0;

The compositions according to the present invention provided animprovement in the general appearance of the skin of volunteers at allevaluated times, being statistically significant (p≤0.05) as compared tothe baseline D0;

In the subjective assessment:

There was an improvement in facial harmony (improvement in the facialcontour) of the volunteers' skin at all evaluated times, also beingstatistically significant (p≤0.05);

There was an improvement in the overall skin appearance (bright,revitalized, rebalanced, healthy looking skin) of the volunteers at allevaluated times, also being statistically significant (p≤0.05);

It was observed that at least 70% and 78% of the volunteers reportedthat facial harmony and overall skin appearance were improved or weregreatly improved, respectively, after using the composition; also beingstatistically significant (p≤0.05);

Example 11 Evaluation of the Increase in Skin Firmness and Elasticity byCutometry for (45+) Day Care Compositions

The methodology consisted of evaluating the increase in skin firmnessand elasticity through cutometry (Cutometer® MPA-580 and MultiprobeAdapter MPA-580, CKeletronics, Germany) performed in the beginning ofthe study and after 28 days of home use of the composition underinvestigation. The increase in skin firmness has been evaluated usingthe Ur/Ue parameter (R5) and the increase in skin elasticity wasevaluated using the Ur/Uf parameter (R7). Cutometry measurements wereperformed on the forearm region and the outer corner of the eye.

25 survey participants have completed the study and their average agewas: 53±4 years, who applied the product on a daily basis once duringdaytime on the face and forearm by spreading it evenly after cleansingthe skin.

Skin firmness has been assessed using the Ur/Ue parameter. Ur/Ueparameter is the ratio between immediate retraction and immediate skindeformation and corresponds to the biological elasticity.

Increased skin firmness is evidenced by an increase in the Ur/Ueparameter that reflects an improvement of properties of the elasticfibers and collagen.

Skin elasticity was evaluated using the Ur/Uf parameter, which consistsof the ratio between immediate retraction and total skin deformation,including the viscous part of skin deformation, and corresponds tobiological elasticity.

Increased skin elasticity is evidenced by the increase in the Ur/Ufparameter that reflects the an improvement of the elastic fiberproperties.

Based on the mean values of the Ur/Ue parameter, the percent increase inskin firmness (% AF) is calculated according to equation 1.

% AFti=100*(Ur/Ueti−Ur/Uet0)/Ur/Uet0   (equation 1)

wherein:

% AFti=percent increase in firmness;

Ur/Ueti=mean values of the Ur/Ue parameter obtained after i days ofstudy (i=28 days);

Ur/Urt0=mean values of the Ur/Ue parameter obtained in the beginning ofthe study (baseline).

Based on the mean values of the Ur/Uf parameter, the percent increase inskin elasticity (% AE) is calculated according to equation 2.

% AEti=100*(Ur/Uf ti−Ur/Uf t0)/Ur/Uf t0   (equation 2)

wherein:

% AEti=percent increase in elasticity;

Ur/Ufti=mean values of the Ur/Uf parameter obtained after i days ofstudy (i=28 days);

Ur/Uft0=mean values of the Ur/Uf parameter obtained in the beginning ofthe study (baseline).

According to the obtained results it could be noted that:

There was a significant increase in skin firmness on the facial regionafter 28 days of home use. This result was evidenced by the significantincrease in the Ur/Ue parameter.

It was found that the compositions according to the present inventionprovided a 36.7% average increase in facial skin firmness after 28 daysof home use as compared to the baseline skin condition.

68% of the survey participants exhibited an increased facial skinfirmness, as evidenced by an increase in the Ur/Ue parameter after 28days of home use of the compositions according to the present invention.

There was a significant increase in skin firmness and elasticity on thevolar forearm region after 28 days of home use. These results wereevidenced by the significant increase in Ur/Ue and Ur/Uf parameters,respectively.

The Ur/Ue parameter has shown that application of the compositionsaccording to the present invention provided a 13.0% average increase inskin firmness on the forearm region and the Ur/Uf parameter has shownthat application of the composition provided a 6.1% average increase inskin elasticity on the forearm region after 28 days of home use ascompared to the baseline skin condition.

72% of the survey participants exhibited increased forearm skinfirmness, as evidenced by an increase in the Ur/Ue parameter after 28days of home use of the composition being investigated.

76% of the survey participants exhibited increased forearm skinelasticity, as evidenced.

FIG. 5 illustrates the mean values of the Ur/Ue parameter obtained infacial and forearm regions in the beginning of the study and after 28days of home use of the compositions of the present invention.

Example 12 Evaluation of the Increase in Skin Firmness and Elasticity byCutometry for (45+) Night Care Compositions

The increase in firmness and elasticity of the skin after 28 days ofhome use of the compositions according to the present invention wasevaluated.

The methodology consisted of evaluating the increase in skin firmnessand elasticity through cutometry (Cutometer® MPA-580 and MultiprobeAdapter MPA-580, CKeletronics, Germany) performed in the beginning ofthe study and after 28 days of home use of the composition underinvestigation. The increase in skin firmness has been evaluated usingthe Ur/Ue parameter (R5) and the increase in skin elasticity wasevaluated using the Ur/Uf parameter (R7). Cutometry measurements wereperformed on the forearm region and on the outer corner of the eye afterapplying the compositions according to the present invention on a dailybasis once during the night-time on the face and forearm by evenlyspreading it after cleansing the skin.

There was no significant increase in skin firmness and elasticity in thefacial region after 28 days of home use. However, 61% of the surveyparticipants exhibited increased skin firmness and 57% exhibitedincreased skin elasticity after 28 days of home use of the compositionsaccording to the present invention.

There was a significant increase in skin firmness and elasticity on thevolar forearm region after 28 days of home use. These results wereevidenced by the significant increase in Ur/Eu and Ur/Uf parameters,respectively.

The Ur/Ue parameter has shown that application of the compositionsaccording to the present invention provided a 8.9% average increase inskin firmness on the forearm region and the Ur/Uf parameter has shownthat application of the compositions according to the present inventionprovided a 5.7% average increase in skin elasticity on the forearmregion after 28 days of home use as compared to the baseline skincondition.

78% of the survey participants exhibited increased forearm skinfirmness, as evidenced by an increased Ur/Ue parameter after 28 days ofhome use of the composition being investigated.

74% of the survey participants exhibited increased forearm skinelasticity, as evidenced by an increased Ur/Uf parameter after 28 daysof home use of the composition being investigated.

Example 13 Evaluation of the Skin Barrier Fortifying Effect Provided bythe Use of (45+) Cosmetic Product

The skin barrier fortifying effect provided by the continuous use of thecosmetic product is evidenced by a reduced water loss observed evenafter removal of stratum corneum layers, exposing the innermost layersof the skin.

Assessment of skin barrier fortification was performed after 7, 14 and28 days of home use of the compositions according to the presentinvention.

26 female survey participants have completed the study and their averageage was: 42±10 years.)

Upon enrollment of the participants in the study, they were instructedto discontinue the use of any topical products on their forearms for 48hours prior to the beginning of the study.

The methodology consisted of assessing the transepidermal water lossfrom the skin after a process of partial removal of the corneous extractin the beginning of the study and after 7, 14 and 28 days of use of thecomposition. A tape-stripping process was used to assess the skinbarrier fortifying effect, where a transparent medical adhesive tape(Transore 3M, 3M, Brazil) was applied and removed 30 times repeatedly onsites marked on the volar forearm, followed by measuring thetransepidermal water loss (Tewameter® 300 and Multiprobe Adapter MPA-5,CKeletronics, Germany). The forearm on which the composition was appliedand the control one were randomly selected. One forearm, which wasidentified with a satin ribbon bracelet, was used for application of thecomposition, while the other forearm remained as control (without theapplication of any products).

The compositions according to the present invention were applied in asufficient amount to the forearm with the satin ribbon bracelet wrappedaround the wrist. The product was applied on the clean forearm once aday at any time.

According to the achieved results, the compositions according to thepresent invention applied to the volar forearm skin provided asignificant effect of skin barrier fortification as compared to thecontrol after 7, 14 and 28 days of home use. The percent value of skinbarrier fortification over the baseline skin condition and the controlwas 22.5% after 7 days, 32.2% after 14 days and 37.3% after 28 days ofhome use. 100.0% of the survey participants exhibited fortification ofthe skin barrier after home use of the investigated composition.

According to the study protocol and procedures used to assess the skinbarrier fortifying effect provided by applying the compositionsaccording to the invention to the forearm skin, it has been found that:

it has provided a significant skin barrier fortification effect ascompared to the control (skin with no products applied) after 7, 14 and28 days of home use.

the percent values of skin barrier fortification achieved over thebaseline skin condition and control were: 22.5% after 7 days, 32.2%after 14 days and 37.3% after 28 days of home use.

100.0% of the survey participants exhibited fortification of the skinbarrier after home use of the investigated composition.

Example 14 Evaluation of Skin Hydration by Corneometry-Compositions(45+)

Skin hydration after application of the compositions according to thepresent invention was assessed.

21 survey participants have completed the study and their average agewas: 45±12 years.) Upon enrollment of the participants in the study,they were asked to discontinue the use of any cosmetic products on theirforearms up to 48 hours prior to the beginning of the study.

For the evaluation two 2.5×4.0 cm sites were marked on the volar forearmof the survey participants, wherein one site was used as control(without any products being applied). After the baseline corneometrymeasurements (Corneometer® 825 and Multiprobe Adapter MPA-5,CKeletronics, Germany), the composition was applied and the surveyparticipants remained in the lab for additional measurements to be takenafter 15 minutes, 4, 6, 8 and 12 hours. After the 12-hour measurementthe survey participants were sent home, being instructed not to wet orwash their arms. The next day they returned to the lab for anothermeasurement to be taken 24 hours after application of the composition.

According to the results obtained, it could be noted that application ofthe compositions according to the present invention kept the skinhydrated for up to 24 hours as compared to the control (skin without anyproducts applied).

Application of the compositions according to the present inventionincreased the skin hydration level by up to 53%. 100% of theparticipants presented an improvement in skin hydration.

Capacitance measurements were performed using the Corneometer® 825 probecoupled to Multi Probe Adapter, MPA 5 (CKeletronics, Germany).

Concomitantly with the measurements, an automated Microsoft® OfficeExcel 2010 software sheet was used to calculate the coefficient ofvariation (CV) of the obtained readings. A minimum of 5 and a maximum of10 measurements were performed per site at each evaluation time. If theCV had a value of less than 6% over 5 measurements, no more measurementswere taken at the site. Otherwise, the readings were continued until aCV of less than 6% was obtained considering a maximum of 10measurements. At the end of 10 measurements, if CV <6% was not achieved,the value of 10% is considered as a new limit to terminate the readingsat the site or to restart the entire process if above 10%.

Skin hydration provided by applying a moisturizing product can beevidenced by an increase in the capacitance value generated in thecapacitor formed between the base of the Corneometer® probe and theskin. The higher the capacitance value, the greater the amount of waterin the skin, and therefore the higher its hydration level.

From the capacitance values (h) the difference in skin hydration (Δh)was calculated, that is, the variation in the capacitance measurementsobtained at each evaluation time over the baseline measurements. The Δhparameter was calculated for the composition and the control, accordingto equation 1.

Δh=hti−ht0   (Equation 1)

From the hydration difference (Δh) values, the parameters of hydration

(H) and percentage of skin hydration (% H) provided by the compositionwere calculated according to Equations 2 and 3.

Hti=Δhti (investigated composition)−Δhti (control)   (equation 2)

Equation 2. Calculation of skin hydration provided by applying thecomposition.

wherein: Hti=skin hydration after i hours from application of thecomposition;

Δhti (control) and Δhti (composition)=differences in skin hydrationobtained for the control and the composition, respectively.

% Hti=(Hti×100)/ht0   (equation 3)

wherein: % Hti=hydration percentage;

Hti=skin hydration provided by applying the composition after i hoursfrom application;

ht0=mean of the capacitance measurements obtained in the beginning ofthe study (baseline).

FIGS. 10 and 11 show the mean hydration values (Hti) and the skinhydration percentage (% Hti) conferred by the application of thecomposition as compared to the control.

According to the study protocol and procedures used to assess skinhydration, it has been found that application of the compositions of thepresent invention on the forearm skin:

conferred significantly higher hydration after 15 minutes, 4, 6, 8, 12and 24 hours from application as compared to control (skin without anyproducts applied). This is an evidence that the investigated compositionmoisturized the skin.

kept the skin moisturized for up to 24 hours after application.

increased the skin hydration level by up to 53%.

100% of the survey participants exhibited increased skin hydration afterapplying the composition.

Example 15 Sensory Analysis to Perceived Efficacy after Application ofthe (60+) Day Care Cosmetic Compositions according to the PresentInvention

Sensory analysis was used to assess the perceived efficacy of thecosmetic compositions according to the present invention.

To that end, 40 survey participants have completed the study and theiraverage age was: 64±3 years; Phototype (Fitzpatrick) 2.5% phototype II,72.5% phototype III and 25.0% phototype IV.

The methodology consisted of a sensory analysis to perceived efficacythrough the application of a questionnaire to be answered by the surveyparticipants of the research after 10 minutes from the application ofthe composition.

After application it was shown that: 100.0% of the survey participantsconsidered that the composition has light texture, leaves the skin softand healthy looking; 97.5% of the survey participants considered thatthe composition provides natural brightness and luminosity to the skin,noticed that the composition leaves an even, homogeneous and smooth skintexture providing a peach skin feel; 95.0% of the survey participantsfound that the composition does not leave the skin oily and providesnatural radiance to the skin; 92.5% of the survey participants perceivedthat the composition revitalizes the skin (reduces the appearance oftired skin); 87.5% of the survey participants perceived that thecomposition recovers the natural radiance of the skin; 82.5% of thesurvey participants perceived that the composition immediately disguisesfine expression lines and wrinkles; 80.0% of the survey participantsperceived that the composition provides a an immediate firmness feel tothe skin (immediate tensor effect).

Example 16 Sensory Analysis to Perceived Efficacy after Application ofthe (60+) Night Care Cosmetic Compositions according to the PresentInvention

Sensory analysis was used to assess the perceived efficacy of thecosmetic compositions according to the present invention.

40 survey participants have completed the study and their average agewas: 64±3 years; Phototype (Fitzpatrick) 5.0% phototype II, 85.0%phototype III and 10.0% phototype IV.

The methodology consisted of a sensory analysis to perceived efficacythrough the application of a questionnaire to be answered by the surveyparticipants of the research after 10 minutes from the application ofthe composition.

After application it was shown that: 100.0% of the survey participantsconsidered that the composition has light texture and provides naturalluminosity to the skin; 97.5% of the survey participants considered thatthe composition provides natural brightness to the skin; 95.0% of thesurvey participants perceived that the composition leaves the skin softand smooth providing a peach skin feel and leaves the skin texture evenand homogeneous; 92.5% of the survey participants perceived that thecomposition provides natural radiance to the skin and gives it a healthyappearance; 90.0% of the survey participants perceived that thecomposition recovers the natural radiance of the skin; 82.5% of thesurvey participants perceived that the composition revitalizes the skin(reduces the tired skin aspect); 80.0% of the survey participantsperceived that the composition does not leave the skin oily; 70.0% ofthe survey participants perceived that the composition provides animmediate firmness sensation to the skin (immediate tensor effect);62,5% of the survey participants perceived that the compositionimmediately disguises fine expression lines and wrinkles.

Example 17 Evaluation of the Anti-Aging Efficacy through InstrumentalMeasurements Under Normal Use Conditions—(60+) Day Care Compositions

The aim of the study was to assess the efficacy of the compositionsaccording to the present invention in reducing wrinkles and improvingskin texture when applied as recommended by means of instrumentalevaluations after 14±2 days, 28±2 days and 56±2 days of use.

The survey participants rested in a temperature- and moisture-controlledroom for 30 minutes before the baseline measurements and during theinterval between measurements. On D0, D14, D28 and D56, 7 consecutiveimages of the periorbital region were obtained using Optical 3D SkinMeasuring Device PRI MOS Compact 5.075 for evaluating wrinkles/textureon one side of the face and 3 (front and side) images using Visia CR(Canfield Scientific, Inc.) that were used for registration purposes.

According to the methodology used to assess efficacy, it was concludedthat relatively to the baseline (D0):

The tested composition caused a reduction in the wrinkle volume aftertwenty-eight and fifty-six days of use;

The tested composition caused a reduction in the average roughness ofthe wrinkles after fourteen, twenty-eight and fifty-six days of use;

The tested composition caused a reduction in the average depth of thewrinkles after fourteen, twenty-eight and fifty-six days of use;

The tested composition caused a reduction in the maximum roughness ofthe wrinkles after fourteen, twenty-eight and fifty-six days of use;

The tested composition caused a reduction in skin undulation aftertwenty-eight and fifty-six days of use;

The tested composition caused a reduction in wrinkle depth aftertwenty-eight and fifty-six days of use;

The tested composition caused an improvement in skin texture aftertwenty-eight and fifty-six days of use.

The results are shown in FIGS. 12 and 13 .

Example 18 Evaluation of the Anti-Aging Efficacy through InstrumentalMeasurements Under Normal Use Conditions—(60+) Night Care Compositions

The aim of the study was to assess the efficacy in reducing wrinkles andimproving skin texture when applied as recommended, by means ofinstrumental evaluations after 14±2 days, 28±2 days and 56±2 days ofuse.

35 participants (female, aged 60 to 69 years, (average age: 64 years),phototypes I to IV, showing wrinkles or expression lines on theperiorbital region and all skin types) were left to rest in atemperature- and moisture-controlled room for 30 minutes before takingthe baseline measurements and in the interval between measurements. OnD0, D14, D28 and D56, 7 consecutive images of the periorbital regionwere obtained using Optical 3D Skin Measuring Device PRI MOS Compact5.075 for evaluating wrinkles/texture on one side of the face and 3(front and side) images using Visia CR (Canfield Scientific, Inc.) thatwere used for registration purposes.

According to the methodology used to assess efficacy, it was concludedthat relatively to the baseline (D0):

The tested composition caused a reduction in the wrinkle volume afterfourteen and fifty-six days of use;

The tested composition caused a reduction in the average roughness ofthe wrinkles after fourteen, twenty-eight and fifty-six days of use;

The tested composition caused a reduction in the average depth of thewrinkles after fourteen, twenty-eight and fifty-six days of use;

The tested composition caused a reduction in the maximum roughness ofthe wrinkles after fourteen, twenty-eight and fifty-six days of use;

The tested composition caused a reduction in skin undulation afterfourteen and fifty-six days of use;

The tested composition caused a reduction in wrinkle depth afterfourteen and fifty-six days of use;

The tested composition caused an improvement in skin texture aftertwenty-eight days of use.

Example 19 Evaluation of the Skin Barrier Fortifying Effect Provided bythe Use of a Cosmetic Product—(60+) Composition

Skin barrier fortification after 7, 14 and 28 days of home use of thecompositions according to the present invention was assessed.

26 survey participants (woman who have completed the study; average age:46±10 years) were assessed as to the transepidermal water loss from theskin after a process of partial removal of the corneous extract in thebeginning of the study and after 7, 14 and 28 days of use of thecomposition.

A tape-stripping process was used to assess the skin barrier fortifyingeffect, where a transparent medical adhesive tape (Transore 3M, 3M,Brazil) was applied and removed 30 times repeatedly on sites marked onthe volar forearm, followed by measuring the transepidermal water loss(Tewameter® 300 and Multiprobe Adapter MPA-5, CKeletronics, Germany).The forearm on which the composition was applied and the control onewere randomly selected. One forearm, which was identified with a satinribbon bracelet, was used for application of the composition, while theother forearm remained as control (without the application of anyproducts).

To do so, application of a sufficient amount of the product on theforearm marked with a satin ribbon bracelet wrapped around the wrist wasrecommended.

According to the achieved results, the composition applied to the volarforearm skin provided a significant effect of skin barrier fortificationas compared to the control after 14 and 28 days of home use. The percentvalue of skin barrier fortification over the baseline skin condition andthe control was 16.0% and 19.3% after 14 and 28 days of home use,respectively. 81% of the survey participants exhibited fortification ofthe skin barrier after home use of the investigated composition.

FIG. 16 illustrates the average variation in transepidermal water lossfrom the skin (ΔEDi) versus time (i=0, 7, 14 or 28 days), obtained forthe investigated composition and the control.

According to the study protocol and procedures used to assess the skinbarrier fortifying effect provided by applying the composition underinvestigation on the forearm skin, it has been found that:

it has provided a significant skin barrier fortification effect ascompared to the control (skin with no products applied) after 14 and 28days of home use.

the percent values of skin barrier fortification achieved over thebaseline skin condition and control were: 16.0% after 14 days and 19.3%after 28 days of home use. 81% of the survey participants exhibitedfortification of the skin barrier after home use of the investigatedcomposition.

Example 20 Evaluation of the Anti-Aging Efficacy of a Cosmetic Productthrough Instrumental Measurements Under Normal Use Conditions—(70+) DayCare Compositions

The aim of the study was to assess the efficacy of the compositionsaccording to the present invention in reducing wrinkles and improvingskin texture when applied as recommended by means of instrumentalevaluations after 14±2 days, 28±2 days and 56±2 days of use.

34 participants (female, aged 70 to 79 years, (average age: 73 years),phototypes I to IV, showing wrinkles or expression lines on theperiorbital region and all skin types) were left to rest in atemperature- and moisture-controlled room for 30 minutes before takingthe baseline measurements and in the interval between measurements. OnD0, D14, D28 and D56, 7 consecutive images of the periorbital regionwere obtained using Optical 3D Skin Measuring Device PRI MOS Compact5.075 for evaluating wrinkles/texture on one side of the face and 3(front and side) images using Visia CR (Canfield Scientific, Inc.) thatwere used for registration purposes.

According to the methodology used to assess efficacy, it was concludedthat relatively to the baseline (D0):

The compositions according to the present invention caused a reductionin the wrinkle volume after fourteen, twenty-eight and fifty-six days ofuse;

The compositions according to the present invention caused a reductionin the average roughness of the wrinkles after fourteen, twenty-eightand fifty-six days of use;

The compositions according to the present invention caused a reductionin the average depth of the wrinkles after fourteen, twenty-eight andfifty-six days of use;

The compositions according to the present invention caused a reductionin the maximum wrinkle roughness after fourteen, twenty-eight andfifty-six days of use;

The compositions according to the present invention caused a reductionin the skin undulation after fourteen, twenty-eight and fifty-six daysof use;

The compositions according to the present invention caused a reductionin wrinkle depth after fourteen, twenty-eight and fifty-six days of use;and

The compositions according to the present invention caused animprovement in skin texture after fourteen, twenty-eight and fifty-sixdays of use.

Example 21 Evaluation of the Anti-Aging Efficacy of a Cosmetic Productthrough Instrumental Measurements Under Normal Use Conditions—(70+)Night Care Compositions

The aim of the study was to assess the efficacy in reducing wrinkles andimproving skin texture when applied as recommended, by means ofinstrumental evaluations after 14±2 days, 28±2 days and 56±2 days ofuse.

35 participants (female, aged 70 to 79 years, average age: 73 years),phototypes I to IV, showing wrinkles or expression lines on theperiorbital region and all skin types) were left to rest in atemperature- and moisture-controlled room for 30 minutes before takingthe baseline measurements and in the interval between measurements.

On D0, D14, d28 and D56, 7 consecutive images of the periorbital regionwere obtained using Optical 3D Skin Measuring Device PRIMOS Compact5.075 for evaluating wrinkles/texture on one side of the face and 3(front and side) images using Visia CR (Canfield Scientific, Inc.) thatwere used for registration purposes.

According to the methodology used to assess efficacy, it was concludedthat relatively to the baseline (D0):

The composition according to the present invention caused a reduction inthe wrinkle volume after fourteen, twenty-eight and fifty-six days ofuse;

The composition according to the present invention caused a reduction inthe average roughness of the wrinkles after fourteen, twenty-eight andfifty-six days of use;

The composition according to the present invention caused a reduction inthe average depth of the wrinkles after fourteen, twenty-eight andfifty-six days of use;

The composition according to the present invention caused a reduction inthe maximum wrinkle roughness after fourteen, twenty-eight and fifty-sixdays of use;

The composition according to the present invention caused a reduction inwrinkle undulation after fourteen, twenty-eight and fifty-six days ofuse; and

The composition according to the present invention caused a reduction inthe wrinkle depth after fourteen and fifty-six days of use.

Example 22 Evaluation of the Skin Barrier Fortifying Effect Provided bythe (70+) Compositions according to the Present Onvention—Day CareCompositions

25 survey participants (woman who have completed the study; average age:42±11 years.) There were no reports or evidence of adverse reactionduring the study.

The methodology consisted of assessing the transepidermal water lossfrom the skin after a process of partial removal of the corneous extractin the beginning of the study and after 7, 14 and 28 days of use of thecomposition. A tape-stripping process was used to assess the skinbarrier fortifying effect, where a transparent medical adhesive tape(Transore 3M, 3M, Brazil) was applied and removed 30 times repeatedly onsites marked on the volar forearm, followed by measuring thetransepidermal water loss (Tewameter® 300 and Multiprobe Adapter MPA-5,CKeletronics, Germany). The forearm on which the composition was appliedand the control one were randomly selected. One forearm, which wasidentified with a satin ribbon bracelet, was used for application of thecomposition, while the other forearm remained as control (without theapplication of any products).

According to the achieved results, the composition applied to the volarforearm skin provided a significant effect of skin barrier fortificationas compared to the control after 7, 14 and 28 days of home use. Thepercent value of skin barrier fortification over the baseline skincondition and the control was 14.5% after 7 days, 21.1% after 14 daysand 25.0% after 28 days of home use. 88.0% of the survey participantsexhibited fortification of the skin barrier after home use of theinvestigated composition.

FIG. 21 shows the average variation in transepidermal water loss fromthe skin obtained in the beginning of the study and after 7, 14 and 28days of use of the (45+) composition over control (Mean±SD, n=25).

Example 23 Evaluation of the Skin Barrier Fortifying Effect Provided bythe Use of a Cosmetic Product—(70+) Night Care Compositions

26 survey participants (woman who have completed the study; average age:44±11 years.)

The methodology consisted of assessing the transepidermal water lossfrom the skin after a process of partial removal of the corneous extractin the beginning of the study and after 7, 14 and 28 days of use of thecomposition. A tape-stripping process was used to assess the skinbarrier fortifying effect, where a transparent medical adhesive tape(Transore 3M, 3M, Brazil) was applied and removed 30 times repeatedly onsites marked on the volar forearm, followed by measuring thetransepidermal water loss (Tewameter® 300 and Multiprobe Adapter MPA-5,CKeletronics, Germany). The forearm on which the composition was appliedand the control one were randomly selected. One forearm, which wasidentified with a satin ribbon bracelet, was used for application of thecomposition, while the other forearm remained as control (without theapplication of any products).

According to the achieved results, the composition applied to the volarforearm skin provided a significant effect of skin barrier fortificationas compared to the control after 7, 14 and 28 days of home use. Thepercent value of skin barrier fortification over the baseline skincondition and the control was 13.0% after 7 days, 18.6% after 14 daysand 27.9% after 28 days of home use. 84.6% of the survey participantsexhibited skin barrier fortification after home use of the composition.

FIG. 22 shows the average variation in transepidermal water loss fromthe skin obtained in the beginning of the study and after 7, 14 and 28days of use of the composition over control (Mean±SD, n=26).

The person skilled in the art, by means of the teachings of the text andexamples disclosed herein, will readily appreciate the advantages of theinvention and will propose equivalent embodiment variations andalternatives without departing from the scope of the invention asdefined in the appended claims.

1.-17. (canceled)
 18. An anti-aging cosmetic composition characterizedin that it is an oil-in-water emulsion comprising: a) at least oneemollient, selected from the group of caprylyl methicone, C12-15 alkylbenzoate, dibutyl adipate, dicaprylyl carbonate, isononyl isononanoate,dicapryl ether, dodecane, ethylhexyl palmitate, ethyl macadamate,isohexadecane, capric/caprylic triglyceride, butters from the Brazilianbiodiversity, isoamyl cocoate or mixtures thereof; b) at least oneantioxidant, selected from the group consisting of butylatedhydroxytoluene (BHT), tocopherol acetate or natural plant extracts, ormixtures thereof; c) at least one humectant, selected from the groupcomprising sugar alcohols, such as sorbitol, mannitol, vegetableglycerin, or mixtures thereof; d) one combination of active ingredientscomprising: Hymenaea courbaril extract and a mixture of sodium cocoylamino acids and sarcosine and potassium aspartate and magnesiumaspartate and propylene glycol; e) at least one emulsifier, selectedfrom the group consisting of glyceryl stearate citrate, potassiumcetylphosphate, PEG-100, acrylates, xanthan gum, cetearyl alcohol,mixture of glyceryl stearate/PEG-100 or mixtures thereof; f) at leastone sensory modifier, selected from the group consisting ofcyclopentasiloxane, dimethicone, cyclopentasiloxane/dimethiconecrosspolymers and titanium isopropyl triisostearate, nylon-12,polymethylsilsesquioxane, aluminum starch octenylsuccinate or mixturesthereof; and g) a cosmetically acceptable carrier.
 19. The compositionof claim 18, characterized in that the butter from the Brazilianbiodiversity is selected from the group consisting of murumuru, cocoa,cupuacu, ucuúba, sapucainha, or mixtures thereof.
 20. The composition ofclaim 18, characterized in that the natural plant extract is Theobromacacao (cocoa).
 21. The composition of claim 18, characterized in that itfurther comprises a viscosity donor selected from a crosspolymer ofacrylate/C₁₀₋₃₀alkyl acrylate, carbopol, a mixture of hydroxyethylacrylate/copolymer of sodium acryloyldimethyltaurate, squalene andpolysorbate 60, or mixtures thereof.
 22. The composition of claim 18,characterized in that it further comprises at least one sunscreen,optionally characterized in that the sunscreen is selected from thegroup consisting of bemotrizinol, diethylaminohydroxybenzoylhexylbenzoate, ethylhexylmethoxy cinnamate, homosalate, bisoctrizole,ethylhexyl triazone or mixtures thereof
 23. The composition of claim 18,characterized in that it further comprises an active ingredient forstimulating skin regeneration selected from Candida saitoana extract,bisabolol, Casearia sylvestris leaf extract and a mixture of Caseariasylvestris/silica or mixtures thereof.
 24. A kit of compositionscharacterized in that it comprises a composition for day-timeapplication and a composition for night-time application, saidcomposition for day-time application being an anti-aging cosmeticcomposition characterized in that it is an oil-in-water emulsioncomprising: a) at least one emollient, selected from the group ofcaprylyl methicone, C12-15 alkyl benzoate, dibutyl adipate, dicaprylylcarbonate, isononyl isononanoate, dicapryl ether, dodecane, ethylhexylpalmitate, ethyl macadamate, isohexadecane, capric/caprylictriglyceride, butters from the Brazilian biodiversity, isoamyl cocoateor mixtures thereof b) at least one antioxidant, selected from the groupconsisting of butylated hydroxytoluene (BHT), tocopherol acetate ornatural plant extracts, or mixtures thereof c) at least one humectant,selected from the group comprising sugar alcohols, such as sorbitol,mannitol, vegetable glycerin, or mixtures thereof; d) one combination ofactive ingredients comprising: Hymenaea courbaril extract and a mixtureof sodium cocoyl amino acids and sarcosine and potassium aspartate andmagnesium aspartate and propylene glycol; e) at least one emulsifier,selected from the group consisting of glyceryl stearate citrate,potassium cetylphosphate, PEG-100, acrylates, xanthan gum, cetearylalcohol, mixture of glyceryl stearate/PEG-100 or mixtures thereof; f) atleast one sensory modifier, selected from the group consisting ofcyclopentasiloxane, dimethicone, cyclopentasiloxane/dimethiconecrosspolymers and titanium isopropyl triisostearate, nylon-12,polymethylsilsesquioxane, aluminum starch octenylsuccinate or mixturesthereof; g) a cosmetically acceptable carrier; and h) at least onesunscreen, optionally characterized in that the sunscreen is selectedfrom the group consisting of bemotrizinol,diethylaminohydroxybenzoylhexyl benzoate, ethylhexylmethoxy cinnamate,homosalate, bisoctrizole, ethylhexyl triazone or mixtures thereof; saidcomposition for night-time application being an anti-aging cosmeticcomposition characterized in that it is an oil-in-water emulsioncomprising: a) at least one emollient, selected from the group ofcaprylyl methicone, C12-15 alkyl benzoate, dibutyl adipate, dicaprylylcarbonate, isononyl isononanoate, dicapryl ether, dodecane, ethylhexylpalmitate, ethyl macadamate, isohexadecane, capric/caprylictriglyceride, butters from the Brazilian biodiversity, isoamyl cocoateor mixtures thereof; b) at least one antioxidant, selected from thegroup consisting of butylated hydroxytoluene (BHT), tocopherol acetateor natural plant extracts, or mixtures thereof; c) at least onehumectant, selected from the group comprising sugar alcohols, such assorbitol, mannitol, vegetable glycerin, or mixtures thereof; d) onecombination of active ingredients comprising: Hymenaea courbaril extractand a mixture of sodium cocoyl amino acids and sarcosine and potassiumaspartate and magnesium aspartate and propylene glycol; e) at least oneemulsifier, selected from the group consisting of glyceryl stearatecitrate, potassium cetylphosphate, PEG-100, acrylates, xanthan gum,cetearyl alcohol, mixture of glyceryl stearate/PEG-100 or mixturesthereof; f) at least one sensory modifier, selected from the groupconsisting of cyclopentasiloxane, dimethicone,cyclopentasiloxane/dimethicone crosspolymers and titanium isopropyltriisostearate, nylon-12, polymethylsilsesquioxane, aluminum starchoctenylsuccinate or mixtures thereof; g) a cosmetically acceptablecarrier; and h) an active ingredient for stimulating skin regenerationselected from Candida saitoana extract, bisabolol, Casearia sylvestrisleaf extract and a mixture of Casearia sylvestris/silica or mixturesthereof.
 25. A non-therapeutic method for cosmetic treatment of theskin, characterized in that it comprises the topical application of acomposition as defined in claim 18 on the skin to be treated, whereinthe application is made during the day and/or at night.
 26. Thenon-therapeutic method of claim 25, characterized in that the skinrefers to the region of the neck, face, arm, forearm, chest and hands.